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. Author manuscript; available in PMC: 2011 Aug 2.
Published in final edited form as: Reproduction. 2010 Nov 12;141(2):227–239. doi: 10.1530/REP-10-0334

Figure 7.

Figure 7

Plasminogen-mediated activation of MMP9. Mouse blastocysts were cultured from GD 7 to GD 9 in medium containing 0, 5, 10, or 20 μg/ml plasminogen, and the conditioned medium was processed by gelatin zymography. (A) Zymograms of human pro-MMP9 (hMMP) and blastocyst-conditioned medium. Conditioned medium (no plasminogen) was treated with APMA to activate MMP9 (APMA). The presence of plasminogen during embryo culture activated MMP9 with a dose dependency. (B) Plasmin activity was measured in medium containing plasminogen (Plg, as indicated) after 2 days of culture either without (M) or with (CM) blastocysts.