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Over-activation of MEK/ERK1/2 partially reverses avocado D003 extract-induced growth inhibition. The 83-01-82CA cells expressing constructively active MEK (MEKCA) and the empty vector (GFP) are described in the Materials and Methods. Cells were treated 0–37.5 µg/ml avocado D003 extract in growth medium for 72 h. Relative cell numbers were determined by the methylene blue assay. The data were normalized to the control treated with vehicle DMSO.
