Hybridization solution | 5 slide pairs | 10 slide pairs | 15 slide pairs | 20 slide pairs |
10xin situ salts | 100 μl | 200 μl | 300 μl | 400 μl |
deionized formamide | 400 μl | 800 μl | 1200 μl | 1600 μl |
50 % Dextran sulfate (+60 °C) | 200 μl | 400 μl | 600 μl | 800 μl |
50x Denhardt's solution | 20 μl | 40 μl | 60 μl | 80 μl |
tRNA (100mg/ml) | 10 μl | 20 μl | 30 μl | 40 μl |
H2O (DEPC treated) | 70 μl | 140 μl | 210 μl | 280 μl |
Total volume | 800 μl | 1600 μl | 2400 μl | 3200 μl |