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. 2009 Apr 17;(26):1205. doi: 10.3791/1205
Hybridization solution 5 slide pairs 10 slide pairs 15 slide pairs 20 slide pairs
10xin situ salts 100 μl 200 μl 300 μl 400 μl
deionized formamide 400 μl 800 μl 1200 μl 1600 μl
50 % Dextran sulfate (+60 °C) 200 μl 400 μl 600 μl 800 μl
50x Denhardt's solution 20 μl 40 μl 60 μl 80 μl
tRNA (100mg/ml) 10 μl 20 μl 30 μl 40 μl
H2O (DEPC treated) 70 μl 140 μl 210 μl 280 μl
Total volume 800 μl 1600 μl 2400 μl 3200 μl