| Ingredient | volume/amount | final concentration |
| MilliQ-water | 585 ml | |
| 10x PBS | 65 ml | 1x PBS |
| 10M NaOH | 520 μl | giving pH ~11 |
| Paraformaldehyde | 26 g | 4% |
| Concentrated HCl | 449 μl | giving pH ~7 |
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