| Ingredient | volume/amount | final concentration |
| Tris (121.14 g/mol) | 60.57 g | 1 M Tris |
| MilliQ-water | up to 500 ml | |
| HCl | giving pH 7.5 | |
| HCl | giving pH 8.0 | |
| NaOH | giving pH 9.5 |
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