| 1x PBS | 30 min |
| 1x PBS | 30 min |
| 30% EtOH | 60 min |
| 40% EtOH | 60 min |
| 50% EtOH | 60 min |
| 60% EtOH | 60 min |
| 70% EtOH | 60 min |
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