| 75% EtOH + 25% Histoclear II | 30 min |
| 50% EtOH + 50% Histoclear II | 30 min |
| 25% EtOH + 75% Histoclear II | 30 min |
| 100% Histoclear II | 60 min |
| 100% Histoclear II | 60 min |
| 100% Histoclear II + ¼ volumes Histowax chip | overnight (no shaking) |
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