Ingredient	volume/amount
10 x NTP labeling mixture	2 μl
10 x Transcription buffer	2 μl
Protector RNase inhibitor	1 μl (20U)
RNA T7 polymerase (SP6 or T3)	2 μl
Template DNA (500-1000 ng)	x μl
RNase- free MilliQ-water	y μl, to a final volume of 18 μl