Table 3.
Effect of PMSG dosage on total number of oocytes
| Strain | PMSG dose(s) | n | No. of oocytes/female mouse (mean ± SE) | Overall P | Pairwise PMSG treatment comparison | Adjusted P |
| C57BL/6 | 2.5 | 20 | 21.5 ± 2.5 | 0.056 | 5: 2.5 | 0.347 |
| 5 | 25 | 26.3 ± 2.6 | 5+5: 5 | 0.466 | ||
| 5+5 | 20 | 30.4 ± 2.1 | 5+5: 2.5 | 0.044 | ||
| B6(Cg)Tyr c-2J/J | 2.5 | 20 | 24.8± 2.5 | <0.001 | 5: 2.5 | 0.824 |
| 5 | 20 | 26.8 ± 3.0 | 5: 2.5+2.5 | <0.001 | ||
| 2.5+2.5 | 20 | 13.1 ± 1.6 | 2.5: 2.5+2.5 | 0.004 | ||
| FVB | 2.5 | 25 | 9.5 ± 1.0 | 0.004 | 5: 2.5 | 0.005 |
| 5 | 25 | 15.1 ± 1.3 | 5: 5+5 | 0.034 | ||
| 5+5 | 24 | 10.7 ± 1.4 | 5+5: 2.5 | 0.776 | ||
| B6D2F1 | 2.5 | 8 | 13.6 ± 3.3 | <0.001 | 5: 2.5 | <0.001 |
| 5 | 8 | 45.3 ± 3.9 | 5: 5+5 | <0.001 | ||
| 5+5 | 7 | 17.9 ± 3.2 | 5+5: 2.5 | 0.683 | ||
| CD1 | 2.5 | 17 | 12.3 ± 1.8 | 0.348 | 5: 2.5 | 0.382 |
| 5 | 17 | 16.3 ± 2.3 | 5+5: 5 | 0.987 | ||
| 5+5 | 18 | 15.8 ± 2.2 | 5+5: 2.5 | 0.459 | ||
| BALB/c | 2.5 | 16 | 21.6 ± 2.9 | <0.001 | 5: 2.5 | <0.001 |
| 5 | 25 | 47.8 ± 3.0 | 5: 5+5 | <0.001 | ||
| 5+5 | 16 | 16.3 ± 2.5 | 5+5: 2.5 | 0.474 |
Female mice of the weight group that gave the best overall results for the strain were given 2.5 or 5 IU PMSG followed 47 to 49 h later by 5 IU HCG. For the third group of mice in each strain, we gave 2.5 or 5 IU PMSG followed by a second dose of 2.5 or 5 IU PMSG 1 wk later and then 5 IU HCG 47 to 49 h after the second dose of PMSG. The overall P value demonstrates whether differences were observed among treatments. The pairwise comparison of dosages and the adjusted P value demonstrate differences between treatments, with the more effective dosage shown first in the comparison.