TABLE 3. Relative Gene Expression Ratios by Microarray and qPCR.
4 h | 16 h | 24 h | |||||
---|---|---|---|---|---|---|---|
Gene | Method | 10 cGy | 2.5 Gy | 10 cGy | 2.5 Gy | 1 Gy | 2.5 Gy |
PTGS2 | Microarray | 3.59 | 3.37 | 0.69 | 0.9 | 3.43 | 3.98 |
qPCR | 2.84 ± 0.21 | 2.57 ± 0.18 | 0.73 ± 0.18 | 1.17 ± 0.32 | 1.64 ± 0.01 | 2.77 ± 0.64 | |
CDK9 | Microarray | 1.3 | 1.38 | 1.57 | 0.91 | 0.86 | 0.9 |
qPCR | 2.80 ± 0.40 | 0.98 ± 0.51 | 2.19 ± 0.11 | 1.48 ± 0.19 | 0.95 ± 0.05 | 1.11 ± 0.01 | |
GPRC5A | Microarray | 2.79 | 3.02 | 2.31 | 1.61 | 1.7 | 1.36 |
qPCR | 1.47 ± 0.12 | 1.82 ± 0.10 | 1.68 ± 0.10 | 1.51 ± 0.17 | 0.86 ± 0.22 | 1.59 ± 0.05 | |
ADAMTS1 | Microarray | 1.51 | 1 | 1.98 | 1.47 | 1.98 | 1.43 |
qPCR | 2.11 ± 0.27 | 1.59 ± 0.04 | 2.25 ± 0.22 | 2.27 ± 0.12 | 1.21 ± 0.47 | 2.04 ± 0.14 | |
ATF3 | Microarray | 1.67 | 1.44 | 1.72 | 1.41 | 1.85 | 0.94 |
qPCR | 2.53 ± 0.42 | 2.67 ± 0.11 | 3.94 ± 0.97 | 1.98 ± 0.64 | 1.03 ± 0.21 | 0.84 ± 0.21 | |
CYP1B1 | Microarray | 4.42 | 2.54 | 1.07 | 0.53 | 1.76 | 0.73 |
qPCR | 3.98 ± 0.13 | 1.98 ± 0.07 | 1.18 ± 0.04 | 2.00 ± 0.22 | 0.81 ± 0.04 | 0.40 ± 0.04 | |
ROR1 | Microarray | 0.53 | 0.95 | 11.98 | 0.85 | 0.63 | 0.69 |
qPCR | 0.55 ± 0.01 | 1.06 ± 0.01 | 4.81 ± 0.07 | 0.49 ± 0.01 | 0.51 ± 0.03 | 0.50 ± 0.02 | |
PSCA | Microarray | 2.74 | 1.83 | 3.44 | 0.97 | 0.52 | 0.67 |
qPCR | 3.20 ± 0.22 | 0.72 ± 0.03 | 2.82 ± 0.10 | 1.78 ± 0.08 | 0.34 ± 0.02 | 0.99 ± 0.02 | |
IGF2 | Microarray | 0.99 | 1.28 | 3.36 | 0.97 | 2.21 | 2.84 |
qPCR | 0.98 ± 0.03 | 1.33 ± 0.05 | 3.30 ± 0.11 | 0.93 ± 0.01 | 2.18 ± 0.03 | 2.68 ± 0.03 | |
APOBEC3A | Microarray | 2.27 | 3.34 | 1.79 | 0.91 | 5.37 | 1.46 |
qPCR | 3.63 ± 0.03 | 5.01 ± 0.05 | 2.15 ± 0.02 | 0.80 ± 0.01 | 2.25 ± 0.05 | 1.23 ± 0.10 | |
BCAS3 | Microarray | 0.67 | 0.72 | 1.35 | 1.63 | 2.78 | 1.04 |
qPCR | 0.47 ± 0.02 | 0.79 ± 0.01 | 1.03 ± 0.01 | 1.30 ± 0.01 | 1.80 ± 0.04 | 1.07 ± 0.01 |