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. 2011 Aug;22(8):1462–1474. doi: 10.1681/ASN.2010121308

Figure 5.

Figure 5.

Knockdown of miR-29b in MEF cells enhances TGF-β1-induced collagen I and III expression but not α-SMA expression. (A) Real-time PCR. (B) Western blotting. The results show that stable knockdown of miR-29b in MEF cells (Smad3WT) significantly enhances TGF-β1 (2 ng/ml)-induced collagen I and III expression but not α-SMA expression. Note that knockdown of miR-29b also significantly enhances constitutive levels of collagen I and III mRNA and protein expression without TGF-β1 stimulation. Each bar represents the mean ± SEM for at least four independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus baseline levels; #P < 0.05, ##P < 0.01, ###P < 0.001 versus control cells. CTL, control.