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. 2011 Aug;22(8):1462–1474. doi: 10.1681/ASN.2010121308

Figure 9.

Figure 9.

Ultrasound-microbubble-mediated miR-29b gene transfer holds progressive renal fibrosis in an established mouse model of UUO nephropathy at day 10. (A) Real-time PCR for miR-29b expression. (B) Masson trichrome stain. (C) Immunohistochemistry for collagen I deposition. (D) Quantitative analysis of collagen I deposition within the UUO kidney. (E) Real-time PCR for collagen I mRNA expression. (F) Western blotting (WB). (G) Quantitative analysis of collagen I expression by Western blotting. The results show that ultrasound-mediated miR-29b gene therapy from day 4 of UUO restores the loss of miR-29b and holds progressive renal fibrosis such as collagen I expression and disposition as determined by histology, immunohistochemistry, real-time PCR, and Western blotting. Each micrograph or bar represents the mean ± SEM for at least six mice. *P < 0.05, ***P < 0.001 versus normal mice; ##P < 0.01, ###P < 0.001 versus control vector (CV)-treated mice or as indicated. Magnification, ×200. IHC, immunohistochemistry.