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. 2011 Aug;22(8):1475–1485. doi: 10.1681/ASN.2010111154

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Copyright © 2011 by the American Society of Nephrology

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Figure 5. — Expression of Induced-by-High-Glucose 1 (IHG-1) regulates expression of mitochondrial proteins manganese superoxide dismutase (MnSOD) and cytochrome (Cyto) c. (A) MnSOD and cytochrome c mRNA were quantified by real-time RT-PCR in HeLa cells expressing tetracycline-inducible IHG-1-specific shRNAi (+ dox) and control cells (− dox). (B) Whole-cell lysates from cells in A were analyzed by immunoblotting with antibodies specific for MnSOD or cytochrome c (panel i). β-Actin demonstrates equal protein loading. Densitometric analysis of Western blots is shown (panel ii). (C) MnSOD and cytochrome c (Cyto c) mRNA was quantified by real-time RT-PCR in HeLa cells overexpressing IHG-1 and control cells (EV). (D) Whole-cell lysates from cells in C were analyzed by immunoblotting with antibodies specific for MnSOD or cytochrome c (panel i). Overexpression of IHG-1 was confirmed by immunoblotting with anti-V5. β-Actin demonstrates equal protein loading. Densitometric analysis of Western blots is shown (panel ii). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (mean ± SEM; n = 3 throughout).