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. 2011 Jul 11;121(8):3176–3188. doi: 10.1172/JCI41695

Figure 2. p120 controls mILC anoikis resistance and tumor growth.

Figure 2

(A) Cells were transduced with viruses carrying mouse p120 shRNAs and grown for 4 days in the absence and presence of anchorage, after which anoikis resistance (n = 4) and growth speed (n = 6), respectively, were analyzed. Western blotting for p120 is shown to depict the extent of shRNA KD in 3 independent mILC cell lines. Akt was used as a loading control. Error bars represent SD of triplicate measurements. *P < 0.001. (B) Luciferase-expressing mILC control shRNA or p120-KD cells were orthotopically transplanted into recipient mice, and tumor volume was measured longitudinally. Error bars represent SEM. *P < 0.05 vs. p120-KD. n = 11 (p120-KD); 16 (control shRNA; of which 12 survived at 7 wk, 10 at 8 wk, 9 at 9 wk). (C) Kaplan-Meier metastasis-free survival plot of the mice in B (n = 11 [p120-KD]; 16 [control shRNA]). Recipient animals were followed in time and sacrificed when bioluminescence was detected in the lungs (>500 counts).