Figure 2. E2 suppresses lipogenesis in ZDF rat islets.

(A and B) Fasting TG (A) and FFA (B) concentrations and area under the curve in male ZL, ZDF, and E2-treated ZDF rats at the indicated time points (n = 4–8). (C) FFA was measured in isolated rat islets at 9 weeks (n = 3–7). (D) TG content in isolated rat islets at 9 weeks (n = 2 [ZL]; 3–4 [ZF and ZDF]). (E) Lipolysis was measured by glycerol release in isolated rat islets at 9 weeks (n = 4–6). (F) Palmitate oxidation was assessed as the release of ³H₂O from (9,10[n]-³H) palmitate in rat islets at 9 weeks (n = 4–7). (G) FA esterification was determined as the incorporation of 1-[¹⁴C]-palmitate into neutral lipids in isolated islets at 9 weeks (n = 3–7). (H) Gene expression of lipogenesis genes in rat islets at 9 weeks, normalized to Actb (n = 2 [ZL]; 4 [ZF and ZDF]). *P < 0.05, **P < 0.01, ***P < 0.001.