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. 2011 Jul 11;121(8):3331–3342. doi: 10.1172/JCI44564

Figure 6. Extranuclear ERs suppress lipid synthesis.

Figure 6

(A) Effect of in vitro E2 treatment (24 hours) on TG content in WT, ERα–/–, ERα+/–, and ERαAA/– islets cultured under lipogenic conditions. ERα–/– islet data were pooled with data from experiments using PERα–/– islets. (B) Effect of in vitro E2 and EDC treatment (both 10–8 M; 24 hours) on TG content in male rat islets cultured under lipogenic conditions. Data are from 4 wells (ZL, ZDF, ZDF + E2) or 2 wells (ZDF + EDC). (C) Effect of E2 and EDC treatment (24 hours) on TG content in INS-1 cells cultured under lipogenic conditions, assessed by TG assay and Nile red staining (n = 1). Original magnification, ×100. (D) Effect of E2 and EDC treatment (4 hours) on Fasn gene expression, normalized to Actb, in INS-1 cells cultured under lipogenic conditions. (E) Effect of E2 and EDC treatment (24 hours) on FAS protein expression in INS-1 cells cultured under lipogenic conditions. (F) Effect of E2 and EDC treatment (24 hours) on FAS enzymatic activity in INS-1 cells cultured under lipogenic conditions. Results are from at least 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. 11 mM (D) or 16 mM (A–C, E, and F) glucose alone.