Figure 1. Isolation of ovarian CA-MSCs.

(A) Photograph of mixed primary ovarian tumor culture (top left) and the resultant, distinct purified colonies of epithelial tumors cells or CA-MSCs. Original magnification, ×100. (B) FACS analysis of primary ovarian tumor ascites, demonstrating CD44⁺CD73⁺CD90⁺ cells. (C) qRT-PCR to confirm CA-MSC purity. CA-MSC lines have no significant expression of the indicated endothelial or epithelial cell markers. Whole primary tumor mRNA (tumor) was used as a positive control. Expression levels were relative to the sample with maximal expression defined as 1. Nml, normal. (D) CA-MSCs demonstrate multipotent differentiation capacity in differential culture conditions. Specific cell stains used were Alizarin Red-S for bone, alcian blue for cartilage, and Oil Red O for adipose. Original magnification, ×100. Pt, patient.