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. 2011 Jul 1;121(8):3206–3219. doi: 10.1172/JCI45273

Figure 5. CA-MSCs have a gene expression profile distinct from that of control MSCs.

Figure 5

(A) Heat map of gene expression of MSC-associated gene expression in CA-MSCs, adipose control MSCs (lot 1, 2, 3), and control MSCs cultured with tumor-conditioned media from either SKOV3 or Hey1 ovarian tumor cells. The blue line indicates genes differentially expressed in CA-MSCs and MSCs treated with tumor-conditioned media. The orange bar indicates genes that appear to be uniquely expressed in CA-MSCs. Avg, average; max, maximum. (B) qRT-PCR using independently derived gene-specific primers performed to confirm differential expression of the indicated genes in control versus 4 CA-MSC–derived cell isolates (top), pooled analysis of qRT-PCR results (middle), and BMP2 expression in controls, cultured CA-MSCs, and FACS-isolated CA-MSCs (bottom). Tum cond media, tumor-conditioned media. (C) Western blot demonstrating increased BMP2 protein expression in adipose control MSCs and CA-MSCs. Quantification of BMP2 band intensity from immunoblotting is shown with average and standard deviation indicated. **P = 0.03. (D) Western blot detection of p-SMAD 1/5 before and after treatment with BMP2 in the presence or absence of the BMP inhibitor Noggin.