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. 2011 Jul 11;121(8):3159–3175. doi: 10.1172/JCI45967

Figure 3. AR positively regulates CCRK transcript and protein expression in liver and HCC cells.

Figure 3

(A) Ectopic AR expression increased CCRK expression. AR and CCRK expression were detected by Western blot following transient transfection. β-actin was used as a loading control. The relative CCRK transcript level was detected in LO2 immortal liver and SK-Hep1 HCC cells by quantitative RT-PCR. GAPDH was used as an internal control. (B) Silencing AR expression downregulated CCRK transcript and protein expression in Huh7 and PLC5 HCC cells. **P < 0.01; ***P < 0.001. (C) Ectopic AR expression induced perinuclear CCRK localization. Double immunofluorescence staining of AR and CCRK was performed in LO2 and SK-Hep1 cells transiently transfected with AR-expressing vector or empty vector. The nuclei were counterstained with DAPI. (D) Localization of AR and CCRK in Huh7 and PLC5 cells following RNA interference. Original magnification, ×400. Data are presented as mean + SD of 3 independent experiments.