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. 2011 Jul 11;121(8):3005–3023. doi: 10.1172/JCI46358

Figure 9. DV effects are mediated via the α5β1 integrin in vivo.

Figure 9

(A) Anti-α5β1 Western blot analysis from PSD 3 mouse brain tissue treated as labeled, with GAPDH as internal control. (B) α5β1 immunohistochemistry of mouse PSD 3 peri-infarct brain tissue with or without DV treatment. Scale bar: 10 μm. (C) Quantification of mean ischemic lesion volumes of stroke WT mice on PSD 1–3 as labeled (*P < 0.05, n = 15 per treatment condition per PSD). (D) Cresyl violet staining, caspase-3 17- to 20-kDa cleavage product immunostaining, and TUNEL staining with PI of peri-infarct brain regions as labeled. Scale bars: 10 μm. (E) Vibrissae-elicited forelimb placement test on WT mice treated as labeled (n = 15 mice per condition from 3 separate experiments with 5 mice each). (F) Von Willebrand factor immunohistochemistry (green) on PSD 5 from WT mice treated as labeled. Scale bar: 10 μm. (G) Peri-infarct blood vessel quantification as labeled (*P < 0.05, **P < 0.01 compared with PBS + IgG on the same day, n = 20 images analyzed per animal, 10 animals per experimental condition). (H) Anti-VEGF Western blot analysis of mouse stroke hemispheres with internal GAPDH as control. (I) Optical density quantification of VEGF Western blot analysis as shown in H (**P < 0.01, n = 5 per experimental condition).