Figure 5.

(A) Reporter gene assay showing that in VSMCs derived from aged Macaca mulatta H₂O₂–induced transcriptional activity of Nrf2 is significantly attenuated as compared with VSMCs derived from young monkeys. Data are mean ± SEM (n = 4–5 for each data point), *p < .05 versus young VSMCs. (B) Expression of NQO1, GCLC (C), and heme oxygenase-1 (D) mRNA in VSMCs derived from young and aged M mulatta. The effects of H₂O₂ (10 μmol/L, for 24 hours) and overexpression of Keap-1 are also shown. Data are mean ± SEM (n = 4–5 in each group; *p < .05).