Fig. 1.

A SDS gradient gel of psoas muscle fibers treated with NEM or DTNB. A rabbit skeletal actin control, Tm rabbit skeletal Tm control; TnT rabbit skeletal troponin T control, Muscle 2 loadings of NEM- or DTNB-treated muscle fibers. The crosslinked Tm region (upper) and the uncrosslinked region (lower) are magnified in B. B Magnified upper and lower regions of the gradient gel of A. Note: (1) presence of α- and β-Tm and absence of crosslinked α-α and α-β Tm for NEM treatment; (2) presence of crosslinked α-α and α-β Tm and absence of uncrosslined α- and β-Tm for DTNB treatment