Figure 4. Decreased phosphorylated S6 and 4E-BP1 levels in CH in the cortex.
(A) Western blot of total (top) and phosphorylated (p) S6 (middle) showing decreased concentrations of pS6 under CH compared to controls. (B) Western blot of total (top) and phosphorylated (p) 4E-BP1 (middle) showing decreased concentrations of p4E-BP1 in CH compared to the controls. (C) and (D) showing significantly decreased concentrations of pS6 compared to total S6 and tubulin respectively. (E) and (F) show significantly decreased concentrations of p4E-BP1 compared to total 4E-BP1 and tubulin respectively. (G) Significantly decreased expression of S6 mRNA expression is observed at all three time points in the CH group (□) from the FACS-sorted GFP-expressing DG progenitor cells (■). **P = 0.001 to 0.01 and *P = 0.01 – 0.05 respectively. Error bars represent standard deviation.