Representative pictures of GFP, BrdU and DCX expression in DG from mice under control (A, B, I) and rapamycin-treated conditions (D, E, J) respectively, showing significantly decreased numbers of GFP-positive (G), BrdU-positive cells (H) and DCX-positive cells (K) with rapamycin treatment as quantified by design-based stereology. The merged picture for control (C) and rapamycin treated (F) shows BrdU-positive cells that are co-localized (arrow) and ones that are not (arrowhead). P-values for GFP, BrdU and DCX are significant. **P < 0.001. Error bars represent standard deviation and scale bars represent 35 μM.