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. Author manuscript; available in PMC: 2011 Aug 2.
Published in final edited form as: Brain Behav Immun. 2008 Dec 16;23(5):649–657. doi: 10.1016/j.bbi.2008.12.002

Fig. 5.

Fig. 5

Gonadectomy increases Th2, Tim-3+ and CTLA-4+ T cells in the heart of males. Mice received 103 PFU CVB3 ip on day 0 after recovery from operations and CD45+ immune cells were isolated from the heart on day 10 pi, during acute myocarditis. FACS analysis depicts expression of Tim-3, CTLA-4 (intracellular) or Foxp3 (intracellular) on/ in CD4+ T cells (A). Intracellular levels of IL-4 (Th2), IFN-γ (Th1) or IL-17 (Th17) were assessed in CD4+ T cells after stimulation for 4 hours with a leukocyte activation cocktail of PMA, Ionomycin and Brefeldin A (B). Similar results were obtained in 2 separate experiments. Data show the mean ±SEM of 5 pooled samples/ group. Each pooled sample consists of cells from 3 mice for a total of 15 mice/ group. *, p < 0.05; **, p ≤ 0.01.