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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Dec 9;1813(2):346–357. doi: 10.1016/j.bbamcr.2010.11.020

Figure 6. The effects of GRP94 overexpression and proteasomal inhibition on the α2C-AR temperature-sensitive trafficking.

Figure 6

A. HEK293T cells were co-transfected with α2C-AR and pcDNA 3.1 (left columns) or GRP94 (right columns). The cell surface α2C-AR levels in cells maintained at 37°C (black columns) or at 30°C for 18 h (white columns) were determined in intact cells as above. mean ± SD, n=9 from three independent transfections. B. α2C-AR tranfected HEK293T cells were incubated with DMSO (vehicle, 0.1%), MG132 (10 μM) or lactacystin (5μM) and subsequently exposed to 30°C for 18h or maintained at 37°C. The α2C-AR plasma membrane levels were determined in intact cells. n=9 from three independent transfections.