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Time courses for NO (25 μM) induced oxidation with human: (A) Hb (B) PEG-LEHs and (C) RBCs. The experimental data shows an average of 8–10 kinetic traces for Hb/PEG-LEHs and 2–3 kinetic traces for RBCs. For experiments with Hb and PEG-LEHs, both NO and Hb, solutions were made in 50 mM Tris buffer (pH 7.4). For experiments with RBCs, NO and RBC, solutions were made in PBS (pH 7.4), to avoid hypotonic shock and lysis of RBCs. The reactions were monitored at 420 nm and 25°C.
