Figure 3.

Spontaneous waves of ATP release in the isolated retina. ATP release was visualized with luciferin-luciferase chemiluminescence in areas of the retina with a high frequency of spontaneous Ca²⁺ waves. A, A chemiluminescence image in x, y coordinates, showing a spontaneous ATP wave at its peak. This image depicts the same spontaneous wave shown in B. B, C, Linescan images of chemiluminescence represented as contour plots of brightness with time shown on the vertical axis. In these two examples, the linescans (illustrated by the red line in A) are centered at the middle of each wave. Like spontaneous Ca²⁺ waves, the ATP waves expand from a central point (at the center/top of each graph) and then shrink, over a total period of 8–15 s.