Table 2.
The profile of proinflammatory cytokine and chemokine secretion of mDCs in response to C. parapsilosis
| mDC (24 h) | |||
|---|---|---|---|
| (pg/ml) | unstimulated | Cp wt | Cp lip-/- |
| IL-1 α | 21.90† (6.64- 70.46) | 241.71 (19.78- 366.12) | 487.97# (110.80- 548.77) |
| IL-6 | 159.26 (38.75- 226.87) | 3934.41 (2481.7-6316.06) | 6535.23# (3122.14-9215.14) |
| TNF α | 99.51 (58.12-158.89) | 1724.67 (736.08-2859.76) | 3454.13# (2934.29-4139.50) |
| CXCL8 | 1632.81 (1358.45-2897.26) | 3420.32 (3268-6563.96) | 2657.64 (1846.33-3076.52) |
| mDC (48 h) | |||
| (pg/ml) | unstimulated | Cp wt | Cp lip-/- |
| IL-1 α | 22.97 (11.17-40.30) | 35.58 (11.19-68.98) | 126.87# (59.90-198.21) |
| IL-6 | 4364.11 (4025.97-5410.58) | 5873.19 (4767.13-7510.32) | 7988.22# (6119.10-9893.27) |
| TNF α | 124.92 (74.93-163.21) | 3456.54 (1628.19-5686.98) | 4345.39 (2694.78-5426.10) |
| CXCL8 | 2223.11 (898.14-4978.58) | 2605.43 (1254.21-5297.94) | 2392.44 (1226.74-5394.56) |
n = 8 independent blood donors
Mature dendritic cells were stimulated with C. parapsilosis wild type (Cp wt), lipase deficient (Cp lip-/-) cells or left unstimulated. Secretion of IL-1α, IL-6, TNF-α or CXCL8 by iDCs was determined by Luminex analyzer or ELISA at 24 h and 48 h post-infection. †: medians (interquartile ranges) # p < 0.05