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. 2011 Jul 12;8:347. doi: 10.1186/1743-422X-8-347

Figure 2.

Figure 2

Expression and purification of recombinant VP5 and VP7. M, Standard protein marker; Lane 1 and Lane 5, empty vector control; Lanes 2-4, VP5 cell lysate extracts induced by IPTG for 1, 3 and 5 h, respectively; Lanes 6-8, VP7 cell lysate extracts induced by IPTG for 1, 3 and 5 h, respectively; Lanes 9-10, purified VP5; Lanes 11-12, purified VP7. All of the total cell extracts and purified proteins were electrophoresed on a 12% SDS-polyacrylamide gel followed by staining with 0.25% Coomassie brilliant blue R-250.