Table 1.
Identification of DENV 1-4 and JEV by the RT-LAMP unit, real-time RT-PCR, and one-step RT-PCR using viral templates at 105-fold dilution
| Viruses | No. of strains | No. of strains correctly identified with primer sets of DENV 1-4 and JEV, respectively | |||
|---|---|---|---|---|---|
| One step RT-PCR | Real-time RT-PCR | RT-LAMP unit | Cross-reaction of RT-LAMP * | ||
| DENV-1 | 17 | 15 | 17 | 17 | 0 |
| DENV-2 | 13 | 11 | 13 | 13 | 0 |
| DENV-3 | 10 | 9 | 10 | 10 | 0 |
| DENV-4 | 12 | 11 | 12 | 12 | 0 |
| JEV | 14 | 12 | 14 | 14 | 0 |
| Influenza viruses** | 12 | 0 | 0 | 0 | nd |
| Hantaviruses** | 12 | 0 | 0 | 0 | nd |
*A given primer set in the RT-LAMP unit was used to amplify other viral templates. ** These viruses were amplified with the primer sets of the five flaviviruses, individually. nd, not done. DENV, dengue virus; JEV, Japanese encephalitis virus; RT-PCR, reverse-transcription polymerase chain reaction; RT-LAMP, reverse-transcription loop-mediated isothermal amplification.