Table 3. Quantitative proteomics analysis of proteins with decreased abundance following glucose starvation.
| Accession Pathema | Accession GI | Ppro | pepCount | mean H/L | Protein Name | Domain of interest |
| EHI_001830 | 183233476 | 1.70E-07 | 6 | 2.00 | hypothetical protein | Gpi16 subunit, GPI transamidase component |
| EHI_109990 | 67472817 | 1.05E-09 | 4 | 2.00 | hypothetical protein | lipoprotein 17 |
| EHI_004610 | 67483002 | 7.30E-04 | 3 | 2.00 | phosphoribulokinase | |
| EHI_185240 | 67481639 | 6.47E-06 | 6 | 2.00 | long chain fatty acid CoA ligase | |
| EHI_196530 | 67465479 | 2.09E-10 | 4 | 2.10 | Ras family GTPase | |
| EHI_121490 | 67463591 | 1.65E-09 | 5 | 2.10 | WD repeat protein | |
| EHI_146330 | 67467663 | 1.44E-14 | 5 | 2.20 | calpain large subunit domain III-containing protein | |
| EHI_011550 | 67474946 | 4.68E-05 | 3 | 2.30 | hypothetical protein | Arrestin WW domains |
| EHI_130930 | 400978 | 2.15E-06 | 5 | 2.30 | Ras-like GTP-binding protein RHO1 | |
| EHI_130930 | 67480401 | 3.40E-07 | 4 | 2.40 | purine nucleoside phosphorylase | |
| EHI_065740 | 183234624 | 7.47E-06 | 4 | 2.40 | HEAT repeat domain-containing protein | |
| EHI_065740 | 67476093 | 6.55E-14 | 6 | 2.40 | 60S ribosomal protein L30 | |
| EHI_096410 | 158932 | 1.52E-08 | 3 | 2.60 | hypothetical protein | |
| EHI_118810 | 67483281 | 3.40E-04 | 3 | 2.00 | hypothetical protein | Protein kinase domain |
| EHI_096410 | 67477219 | 2.60E-06 | 3 | 2.70 | protein kinase domain-containing protein | |
| EHI_012170 | 67481683 | 2.91E-04 | 3 | 2.90 | hypothetical protein | von Willebrand factor |
| EHI_127830 | 67479381 | 6.51E-05 | 4 | 2.90 | long chain fatty acid CoA ligase | |
| EHI_168240 | 67469932 | 1.22E-14 | 5 | 3.10 | cysteine proteinase –A5 | |
| EHI_159480 | 67475078 | 1.17E-06 | 6 | 4.30 | pore-forming peptide ameobapore A precursor | |
| EHI_194540 | 183231856 | 9.24E-08 | 4 | 6.30 | pore-forming peptide ameobapore B precursor |
Total lysates of control and glucose-starved E.histolytica trophozoites were produced and used as substrates for stable isotope dimethyl labeling of peptides for quantitative proteomics analysis. The list describes the proteins that were up-regulated (Table 2) or down-regulated (Table 3) following glucose starvation. Mean H/L provided directly from the MS analysis is the ratio between the protein levels in lysates from glucose-starved and control trophozoites. The value displayed for pepCount represents the number of peptides used for protein identification and statistical value is given in the column Ppro (protein probability). Ppro is the probability of the best peptide match (the peptide with the lowest score) for each identified protein. Classification and characterization of the domains in the hypothetical proteins was done using motif Search (http://motif.genome.jp/) of the PROSITE and Pfam databases.