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. 2011 Aug 2;5(8):e1262. doi: 10.1371/journal.pntd.0001262

Figure 1. PCR-RFLP analysis of Giardia isolates.

Figure 1

Panel A: Gelred (Biotium) stained 3.5% MetaPhor agarose gel (Cambrex) showing electrophoretic separation of nested β-giardin PCR products (511 bp) after digestion with HaeIII: lane 1, assemblage A (novel pattern Sweh166, 173, 178); lane 2, assemblage A (ordinary pattern); lane 3, assemblage B (ordinary pattern), lane 4, assemblage B (novel pattern Sweh198); lane 5, assemblage B (mixed pattern). Panel B: Electrophoretic separation of nested tpi PCR products (530 bp) after digestion with DdeI: lane 1, assemblage A; lane 2, assemblage B (ordinary pattern); lane 3, assemblage B (novel pattern Sweh121), lane 4, assemblage B (novel pattern Sweh154); lanes 5, 6 and 7, assemblage B (mixed patterns). Panel C: Electrophoretic separation of semi-nested gdh PCR products (432 bp) after digestion with NlaIV: lane 1, assemblage AII; lane 2, assemblage AI; lanes 3 and 4, assemblage B; lanes 5 and 6, assemblage B (mixed patterns). Molecular size markers (M) are 50-bp ladders (Invitrogen).