Table 4. Bayesian estimates of divergence time (in mya) for different T. cruzi lineages.

Nuclear loci (20 loci)
Clockmodel	Tryps a	T. cruzi b	TcI c	TcI, TcIII-VI d	TcII-Hybrids f (TcV, TcVI)	TcIII-Hybrids f (TcV, TcVI)	PosteriorLikelihood
Strict	6.23(4.7–7.7)	1.36(1–1.7)	0.15(0.09–0.2)	1.11(0.8–1.4)	0.49(0.3–0.6)	0.49(0.3–0.6)	−57232.7477
Relaxedlognormal	8.3(3.8–13.8)	2.18(0.9–3.7)	0.25(0.08–0.5)	1.69(0.77–2.9)	0.8(0.3–1.4)	0.73(0.3–1.3)	−57183.9809

Times to the most recent common ancestor (tMRCA) are shown in mya. In parentheses are 95% HPD (highest posterior density) intervals.

^atMRCA of T. cruzi and its two outgroups (T. c. marinkellei, T. verspertilionis).

^btMRCA of extant T. cruzi lineages.

^ctMRCA of TcI (nuclear data: SO34, SC13; mtDNA: TEH cl2, CEPA EP, Vin C6, X10 cl1, SABP3, A80, A92, MA-V, OPS21 cl11, CUTIA cl1, 133 79 cl7, V121, 26 79, CUICA cl1, SO34 cl4, P209 cl1, 85/818, P0AC, Esquilo cl1, SC13).

^dtMRCA of strains SO34, SC13, CL35, EP225, CLA39-Haplotype1 and CL_Brener-Haplotype1.

^etMRCA of strains Florida C16, CANIII, M6241, CM 17, EP 255, 86-1, SO3, EPP, PSC-O, Tulahuen, CL F11F5, VM V4, P63, 86/2036, P251, X9/3, XII0/8 and XI09/2.

^ftMRCA of TcII or TcIII and the respective closest haplotypes from both hybrid DTUs (TcV, TcVI).

^gtMRCA of strains Esmeraldo, X-300, CBB, MCV, MSC2, TU18, MVB.

^hThe Relaxed Lognormal clock model for the mitochondrial data set did not converge even after combining 10 independent runs in Tracer. Therefore, the estimates from these analyses are not reliable and not shown here.