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. 2011 Aug 2;6(8):e22635. doi: 10.1371/journal.pone.0022635

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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PBMC from healthy donors were incubated for 72 h with medium only, GRFT or CV-N at 788 nM and 182 nM, respectively. Supernatants were collected and cytokine levels were measured by the Bio-Plex array system. The fold-increase values of the cytokine concentrations in the supernatant of stimulated PBMC with respect to the concentrations in the supernatant of untreated cells were determined from 4 and 11 different donors for GRFT and CV-N, respectively. The fold-increase values are divided in subgroups: 1–3 fold-increase (white squares), 3–10 fold-increase (yellow squares), 10–100 fold-increase (orange squares), 100–500 fold-increase (dark red squares) and >500 fold-increase (black squares). The amount of fold-increase values for each chemokine/cytokine is given as percentage in the total amount of donors (Y-axis).