Figure 7. Tumor exosomes inhibit BMDC maturation and induce TGF-β1 production.

(A) Day 8 BMDCs (purity >90%) were treated with 10 µg/ml of tumor exosomes or cultured untreated for 3 days. The expression of I-A^b and CD86 were analyzed by FACS. LPS treatment (1 µg/ml) for 24 h was used as a DC maturation control. (B) TGF-β1 protein levels (pg/ml) in DC culture supernatants after exosome treatment. Data show the mean values of two independent experiments ± SD. (C) TGF-β1 contents in exosome preparations (pg/10 µg of exosomes). For each exosome sample, the data shown represent the mean value of three preparations ± SD.