Skip to main content
. 2011 Aug 2;6(8):e23135. doi: 10.1371/journal.pone.0023135

Figure 3. B. abortus induced-apoptosis depends on type I IFN signaling.

Figure 3

(A) Spleens from 129Sv/Ev or IFN-αβR KO mice after two-weeks of infection with B. abortus were removed and tissue sections were analyzed to determine the apoptotic cells by TUNEL assay as described in material and methods. (B) This graph demonstrates the % of TUNEL-positive cells shown in panel A. (C) Annexin V+/7-AAD− analysis of spleen cells of Brucella-infected 129Sv/Ev or IFN-αβR KO mice performed by flow cytometry. (D) The expression levels of TRAIL were measured by real-time PCR in spleens of Brucella-infected 129Sv/Ev or IFN-αβR KO mice. Similar results were obtained in two-independent experiments. Error bars represent the mean ±SD. Statistically significant difference from Brucella-infected and uninfected mice is denoted by an asterisk and between IFN-αβR KO mice compared to wild type is denoted by two asterisks (p<0.05).