Fig. 5.

Effects of P11 on activities of MAP kinases and transcription factors in bFGF-induced HUVECs. A, HUVECs were preincubated with or without P11 (10 μg/ml, 15 μm) for 30 min and then stimulated with bFGF (3 ng/ml). At the indicated time after bFGF stimulation, the cell lysates were analyzed by Western blotting for the phosphorylation of MEK and ERK1/2. B, Activity assay of various transcription factors in bFGF-induced HUVECs treated with or without P11. HUVECs were preincubated with P11 (10 μg/ml) for 30 min, followed by stimulation with bFGF for 30 min. Activation of transcription factors was analyzed using a BD™ TransFactor profiling Kit (Becton Dickinson Science). The histograms represent the relative intensities of the transcription factors activities as determined by colorimetric analyses. The data show the means of three independent experiments (mean ± S.E.).