Table III. Identification of Schistosoma japonicum schistosomulum proteins differentially expressed between rats and mice: MALDI-TOF MS analyses. Schistosomula were recovered from the hosts 10 days after infection with cercariae. All the identified proteins matched with those in Schistosoma japonicum database.

Spot IDa	Database ID no.	Identified protein name	MASCOT score	Sequence coverage (%)b	Theoretical Mr/pIc	Functional clusteringd	Fold changee
312	gi 56753427	similar to heat shock 70kDa protein 9B	625	43	71252/6.25	Response to stimulus/stress	1.25
388	gi 56757978	similar to Pkm2 protein	182	27	61146/6.48	carbohydrate metabolic process	1.23
435	gi 226468624	putative protein disulfide isomerase-associated 3 precursor	353	34	54671/6	protein transport	1.3
500	gi 226471566	similar to p50 immunophilin	146	33	48551/5.65	Response to stimulus/stress	1.36
723/729	gi 56754181	pyruvate dehydrogenase E1 alpha 1	259/485	24/47	43940/8.44	carbohydrate metabolic process	1.31/1.45
1268	gi 226489438	Thioredoxin peroxidase	233	28	21564/5.93	regulation of gene expression	1.64
1351	gi 226475504	putative cold shock domain protein A	125	21	22381/10.63	RNA metabolic process	1.76
1558	gi 76163073	similar to cysteine protease inhibitor	104	51	10026/8.53	protein metabolic process	1.26
1578	gi 171473822	similar to heat shock 10kD protein 1	191	67	12074/5.88	protein metabolic process	2.25
347	gi 76154176	similar to matricin	255	32	24343/9.41	protein metabolic process	−1.27
505	gi 56759094	similar to Heterogeneous nuclear ribonucleoprotein K	80	23	42176/8.61	RNA metabolic process	−1.62
524	gi 462011	Enolase	130	17	47221/6.18	carbohydrate metabolic process	−1.83
578/590	gi 226473300	Mitochondrial import receptor subunit TOM34	77/72	17/17	30253/5.02	protein metabolic process	−3.03/−2.15
585	gi 76156278	similar to Mitochondrial import receptor subunit TOM34	82	14	27877/4.78	protein metabolic process	−2.46
602	gi 56756857	similar to DEAD box ATP-dependent RNA helicase	299	35	46113/6.16	RNA metabolic process	−1.37
694	gi 60688010	similar to heat shock cognate protein	206	52	9282/9.76		−1.68
707	gi 226467798	actin 5C	156	26	41706/5.3	cytoskeleton organization	−1.29
790	gi 226475588	phosphoglycerate kinase 1	197	25	44253/6.76	carbohydrate metabolic process	−1.80
852	gi 226478690	Tropomyosin	108	35	33006/4.63		−1.84
853/900	gi 226473592	aldo-keto reductase family 1, member B4 (aldose reductase)	113/64	20/23	35612/6.87	carbohydrate metabolic process	−1.53/−1.50
1013	gi 226472458	carbonyl reductase 1	199	54	30647/6.36	carbohydrate metabolic process	−1.55
1180	gi 62738608	Chain A, Orthorhombic Glutathione S-Transferase	329	53	26018/6.09	protein metabolic process	−1.58
1212	gi 226487138	proteasome subunit beta type 4	96	46	27877/5.92	protein metabolic process	−2.02
1230	gi 226478722	Tegument antigen (I(H)A)	591	62	21679/6.45		−1.29
1246	gi 226479412	tryparedoxin peroxidase	313	51	20776/6.42	protein metabolic process	−1.25
1292	gi 226472634	similar to preprocathepsin C	287	26	52787/8.76	protein metabolic process	−1.66

^a The spot ID was determined at the beginning of analysis of gels.

^b The percentage coverage was defined as the ratio (%) of the protein sequence covered by the matched peptides.

^c Theoretical M_r/pI was calculated using DNAStar software.

^d The functions are classified by gene ontology (GO) annotations using Blast2GO and AmiGO.

^e Fold change is calculated by DeCyder software (Version 6.5).