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Reciprocal association of FBL, Nop52, Guα, Guβ, or NCL with p32. Proteins were immunoprecipitated with anti-FLAG from the whole-cell extract of 293EBNA cells expressing FLAG-tagged FBL (A), Nop52 (A), Guα (B), Guβ (C), or nucleolin (NCL) (D), and detected by immunoblotting (IB) with anti-FBL, anti-Nop52, anti-FLAG or anti-p32 before (–) and after (+) treatment with RNase A. Input: 30 μg of an aliquot of whole-cell extract.
