Skip to main content
. 2011 May 18;10(8):M111.008128. doi: 10.1074/mcp.M111.008128

Table II. Extracellular matrix and associated proteins identified by proteomics in the 4 m guanidine extracts. aThree biological samples performed with two technical replicates each (n = 6). bFour biological samples performed with two technical replicates each (n = 8). cIndicates proteins identified for the first time by proteomics in the vasculature. ND: none detected.

Protein name Swiss Prot Accession MW (kDa) Mean spectra±S.E. CONa Mean spectra±S.E. AAAb p value (t-test)
Proteoglycans and glycoproteins
    Fibronectin FINC_HUMAN 263 210 ± 15 336 ± 16 ≥0.0001
    Perlecan PGBM_HUMAN 469 323 ± 13 176 ± 43 0.0280
    Versican CSPG2_HUMAN 373 190 ± 5 84 ± 18 0.0010
    Aggrecan PGCA_HUMAN 250 154 ± 23 28 ± 11 0.0003
    Prolargin PRELP_HUMAN 44 76 ± 8 86 ± 8 0.3100
    Tenascin TENA_HUMAN 241 11 ± 1 144 ± 21 ≥0.0001
    Biglycan PGS1_HUMAN 42 74 ± 4 78 ± 10 0.4400
    Vitronectin VTNC_HUMAN 54 26 ± 2 117 ± 12 ≥0.0001
    Decorin PGS2_HUMAN 40 35 ± 7 70 ± 14 0.0430
    Clusterin CLUS_HUMAN 52 34 ± 2 69 ± 7 ≥0.0001
    Periostin POSTN_HUMAN 93 56 ± 6 47 ± 6 0.6400
    Lactadherin MFGM_HUMAN 43 60 ± 12 39 ± 11 0.2400
    Lumican LUM_HUMAN 38 41 ± 2 51 ± 6 0.1400
    Thrombospondin-1 TSP1_HUMAN 129 2 ± 1 90 ± 12 ≥0.0001
    Tenascin-X TENX_HUMAN 464 31 ± 1 39 ± 10 0.3600
    Nidogen-1 NID1_HUMAN 136 29 ± 4 24 ± 7 0.6700
    Aortic carboxypeptidase-like protein AEBP1_HUMAN 131 21 ± 2 28 ± 6 0.2900
    Laminin subunit gamma 1 LAMC1_HUMAN 178 26 ± 6 16 ± 6 0.3300
    Mimecan MIME_HUMAN 34 15 ± 2 23 ± 8 0.3200
    Laminin subunit beta 2 LAMB2_HUMAN 196 23 ± 4 12 ± 4 0.0960
    Dermatopontin DERM_HUMAN 24 17 ± 5 16 ± 3 0.8100
    Annexin A2 ANXA2_HUMAN 39 13 ± 2 19 ± 5 0.2600
    Hyaluronan and proteoglycan link protein 1 HPLN1_HUMAN 40 25 ± 9 6 ± 2 0.0510
    Fibrillin-1 FBN1_HUMAN 312 4 ± 1 26 ± 4 ≥0.0001
    Laminin subunit alpha 5 LAMA5_HUMAN 400 19 ± 4 6 ± 2 0.0060
    Fibulin-5 FBLN5_HUMAN 50 14 ± 3 3 ± 1 0.0110
    Metalloproteinase inhibitor 3 TIMP3_HUMAN 24 3 ± 1 14 ± 3 0.0067
    Galectin-1 LEG1_HUMAN 15 9 ± 3 8 ± 2 0.9900
    Asporin ASPN_HUMAN 43 2 ± 1 12 ± 5 0.0800
    EMILIN-1 EMIL1_HUMAN 107 8 ± 3 3 ± 1 0.1700
    Microfibril-associated glycoprotein 4 MFAP4_HUMAN 29 9 ± 2 2 ± 1 0.0120
    Nidogen-2 NID2_HUMAN 151 2 ± 0 7 ± 3 0.0630
    Fibromodulin FMOD_HUMAN 43 5 ± 2 3 ± 0 0.3700
    Fibulin-1 FBLN1_HUMAN 77 6 ± 2 1 ± 0 0.0110
    Agrin AGRIN_HUMAN 215 4 ± 2 3 ± 1 0.5700
    Thrombospondin-2 TSP2_HUMAN 130 ND 7 ± 1 ≥0.0001
    Microfibrillar-associated protein 5 MFAP5_HUMAN 20 3 ± 1 3 ± 1 0.5400
    Glia-derived nexin GDN_HUMAN 44 ND 4 ± 1 0.0014
    Laminin subunit beta 1 LAMB1_HUMAN 198 ND 4 ± 1 0.0110
    RPE-spondin RPESP_HUMAN 30 4 ± 2 1 ± 1 0.0330
    Podocan PODN_HUMAN 69 ND 3 ± 1 0.0700
    Matrix Gla proteinc MGP_HUMAN 12 1 ± 0 1 ± 1 0.7500
    Sclerostin SOST_HUMAN 24 1 ± 0 ND 0.1800
    SPARC-like protein 1c SPRL1_HUMAN 75 1 ± 1 ND 0.1100
    Cartilage oligomeric matrix protein COMP_HUMAN 83 1 ± 1 ND 0.0930
    Matrilin-2 MATN2_HUMAN 107 1 ± 0 1 ± 1 0.7900
    Galectin-3 LEG3_HUMAN 26 1 ± 1 ND 0.2700
    Spondin-1 SPON1_HUMAN 91 1 ± 1 ND 0.1300
    Cartilage acidic protein 1c CRAC1_HUMAN 71 ND 1 ± 1 0.2100
    SPARC-related modular calcium-binding protein 2 SMOC2_HUMAN 50 1 ± 0 ND 0.3200
    Fibronectin type III domain-containing protein 1 FNDC1_HUMAN 205 1 ± 0 ND 0.2300
    Laminin subunit alpha 4 LAMA4_HUMAN 203 1 ± 0 ND 0.3000
    Hemicentin-1 HMCN1_HUMAN 613 ND 1 ± 0 0.4100
ECM-associated proteins
    Serum amyloid P component SAMP_HUMAN 25 39 ± 6 91 ± 20 0.0220
    Apolipoprotein B-100 APOB_HUMAN 516 ND 96 ± 34 0.0470
    Apolipoprotein A-I APOA1_HUMAN 31 ND 50 ± 16 0.0230
    Serine protease HTRA1 HTRA1_HUMAN 51 16 ± 4 19 ± 7 0.7600
    TGF-beta induced protein ig-h3 BGH3_HUMAN 75 8 ± 3 27 ± 4 ≥0.0001
    Insulin-like growth factor-binding protein 7 IBP7_HUMAN 29 20 ± 2 9 ± 3 0.0430
    EGF-containing fibulin-like extracellular matrix protein 1 FBLN3_HUMAN 55 13 ± 2 12 ± 5 0.9900
    Cathepsin D CATD_HUMAN 22 8 ± 2 14 ± 4 0.1090
    Extracellular superoxide dismutase SODE_HUMAN 26 18 ± 3 5 ± 2 0.0020
    Target of Nesh-SH3 TARSH_HUMAN 119 20 ± 4 3 ± 1 0.0002
    Metalloproteinase inhibitor 3 TIMP3_HUMAN 24 3 ± 1 14 ± 3 0.0067
    Apolipoprotein E APOE_HUMAN 36 ND 15 ± 4 0.0180
    Macrophage metalloelastasec MMP12_HUMAN 54 ND 15 ± 3 0.0033
    Apolipoprotein D APOD_HUMAN 21 ND 13 ± 2 0.0035
    Latent-transforming growth factor beta-binding protein 2 LTBP2_HUMAN 195 9 ± 2 4 ± 1 0.0570
    Latent-TGF-beta binding protein, isoform 1S LTB1S_HUMAN 153 8 ± 2 4 ± 2 0.3000
    Ceruloplasmin CERU_HUMAN 122 ND 12 ± 5 0.0650
    Transforming growth factor beta-1-induced transcript 1 protein TGFI1_HUMAN 50 10 ± 2 1 ± 1 0.0003
    Cathepsin G CATG_HUMAN 29 3 ± 2 7 ± 2 0.0450
    Chymase CMA1_HUMAN 27 7 ± 1 3 ± 2 0.2700
    Myeloperoxidase PERM_HUMAN 84 ND 10 ± 3 0.0210
    Neutrophil defensin 1 DEF1_HUMAN 10 1 ± 1 9 ± 1 0.0003
    LIM and cysteine-rich domains protein 1 LMCD1_HUMAN 41 9 ± 2 ND 0.0067
    Lysyl oxidase homolog 1 LOXL1_HUMAN 63 5 ± 2 3 ± 1 0.2900
    Galectin-3-binding protein LG3BP_HUMAN 65 6 ± 3 2 ± 0 0.1000
    Tryptase beta-1 TRYB1_HUMAN 31 4 ± 1 3 ± 2 0.9900
    Metalloproteinase inhibitor-3 TIMP1_HUMAN 23 ND 6 ± 2 0.0270
    Pigment epithelium-derived factor PEDF_HUMAN 46 2 ± 1 3 ± 1 0.6100
    Carboxypeptidase-like protein X2 CPXM2_HUMAN 86 4 ± 2 ND 0.1600
    Apolipoprotein A-IV APOA4_HUMAN 45 ND 4 ± 3 0.1900
    Phospholipase A2, membrane associated PA2GA_HUMAN 16 2 ± 1 2 ± 1 0.9300
    Apolipoprotein L1 APOL1_HUMAN 44 ND 4 ± 2 0.1900
    Protein S100-A9c S10A9_HUMAN 13 ND 4 ± 1 0.0290
    Latent-transforming growth factor beta-binding protein 4 LTBP4_HUMAN 173 3 ± 1 ND 0.0550
    Procollagen C-endopeptidase enhancer 1 PCOC1_HUMAN 48 2 ± 1 ND 0.1700
    Kallistatin KAIN_HUMAN 49 ND 2 ± 2 0.3300
    Properdin PROP_HUMAN 51 1 ± 1 1 ± 0 0.7500
    Calreticulin CALR_HUMAN 48 1 ± 1 1 ± 0 0.2900
    Antileukoproteinase SLPI_HUMAN 14 1 ± 0 ND 0.0980
    Cathepsin Z CATZ_HUMAN 34 1 ± 1 ND 0.4800
    Secreted frizzled-related protein 1 SFRP1_HUMAN 35 2 ± 1 ND 0.1200
    SPARC-like protein 1 SPRL1_HUMAN 75 1 ± 1 ND 0.1100
    Protein S100-A8c S10A8_HUMAN 11 1 ± 0 1 ± 0 0.4200
    Secreted frizzled-related protein 3 SFRP3_HUMAN 36 1 ± 1 ND 0.1400
    C-type lectin domain family 11 member Ac CLC11_HUMAN 36 1 ± 0 1 ± 0 0.9500
    Lipocalin 1c LCN1_HUMAN 19 ND 1 ± 0 0.1800
    Secreted phosphoprotein 24 SPP24_HUMAN 24 ND 1 ± 1 0.1600
    EGF-like repeat and discoidin I-like domain-
        containing protein 3 EDIL3_HUMAN 54 ND 1 ± 1 0.2400
    Insulin-like growth-factor binding protein complex acid labilec ALS_HUMAN 66 ND 1 ± 0 0.2900
Collagens
    Collagen alpha 3(VI) CO6A3_HUMAN 344 194 ± 35 343 ± 37 0.0025
    Collagen alpha 1(VI) CO6A1_HUMAN 109 74 ± 10 95 ± 16 0.1100
    Collagen alpha 1(XIV) COEA1_HUMAN 194 112 ± 17 40 ± 9 0.0005
    Collagen alpha 1(XVIII) COIA1_HUMAN 178 69 ± 6 13 ± 4 ≥0.0001
    Collagen alpha 1(I) CO1A1_HUMAN 139 36 ± 5 41 ± 2 0.0580
    Collagen alpha 1(XII) COCA1_HUMAN 333 17 ± 3 60 ± 10 0.0006
    Collagen alpha 2(VI) CO6A2_HUMAN 109 35 ± 6 40 ± 7 0.2400
    Collagen alpha 2(I) CO1A2_HUMAN 129 34 ± 2 36 ± 2 0.0580
    Collagen alpha 2(IV) CO4A2_HUMAN 168 37 ± 6 31 ± 12 0.7700
    Collagen alpha 1(XV) COFA1_HUMAN 142 24 ± 6 17 ± 5 0.4300
    Collagen alpha 1(V) CO5A1_HUMAN 184 8 ± 1 11 ± 2 0.0300
    Collagen alpha 1(VIII) CO8A1_HUMAN 73 7 ± 2 9 ± 3 0.6200
    Collagen alpha 2(VIII) CO8A2_HUMAN 67 2 ± 1 3 ± 1 0.3100
    Collagen alpha 1(IV) CO4A1_HUMAN 161 1 ± 1 2 ± 1 0.4700
    Collagen alpha 1(XXI) COLA1_HUMAN 99 1 ± 0 2 ± 1 0.2100
    Collagen alpha 2(V) CO5A2_HUMAN 145 ND 1 ± 0 0.0820