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siRNA transfection effectively reduces expression of Fis1 and Drp1. HAEC’s were transfected with Fis1 or Drp1 siRNA for 48 hours as described in Methods. siRNA transfection reduced message (A and B) and protein (C and D) in HAEC’s exposed to 5 mM or 30 mM glucose (interaction P<0.01 by ANOVA; *P<0.05 for 5 mM versus 30 mM glucose and #P<0.05 for siRNA versus control by SNK pairwise comparison). Data are mean ± SEM for 3 experiments
