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. Author manuscript; available in PMC: 2012 Jul 26.
Published in final edited form as: Circulation. 2011 Jul 11;124(4):444–453. doi: 10.1161/CIRCULATIONAHA.110.014506

Figure 6.

Figure 6

Silencing Fis1 or Drp1 expression prevents glucose-induced loss of mitochondrial networks and glucose-induced ROS production. A. Mitochondria were imaged in HAEC’s with Mitotracker Green and representative images show that silencing either fission protein maintained mitochondrial networks under high glucose conditions. Pooled data show a protective effect on network extent (interaction P<0.001 by ANOVA). B. Mitochondrial ROS production was imaged in HAEC’s with Mitosox and representative images show that silencing either fission protein produces a marked decrease in ROS production. Pooled data confirm that Fis1 and Drp1 siRNA prevent glucose-induced increases in ROS (interaction P<0.001 by ANOVA; *P<0.001 versus 5 mM glucose and #P<0.01 versus control by SNK pairwise comparison). Data are mean ± SEM for 3 experiments.