Skip to main content
. Author manuscript; available in PMC: 2012 Jul 26.
Published in final edited form as: Circulation. 2011 Jul 11;124(4):444–453. doi: 10.1161/CIRCULATIONAHA.110.014506

Figure 7.

Figure 7

Silencing Fis1 or Drp1 expression prevents glucose-induced impairment of eNOS activation. As described in Methods, eNOS phosphorylation was measured after 5 minute exposure to acetylcholine (A), insulin (B), or A23187 (C) in the presence of 5 or 30 mM (high) glucose. Silencing either fission protein restored the eNOS phosphorylation response to each agonist with 30 mM glucose (interaction P<0.001 by ANOVA; *P<0.01 compared to control by SNK pairwise comparison). Addition of tiron (5mM) also restored the response to A23187 with 30 mM glucose, and the combination of siRNA and tiron had no additional effect. Data are shown as mean ± SEM for 3 experiments.