Figure 4.

TCR CDR3β heteroduplex analysis of bovine CD8⁺ T-cell responses to T. parva: Results obtained for each Vβ subgroup are shown for CD8⁺ T cells harvested ex vivo 10 days after parasite challenge of animal 592 (A) and with a CD8⁺ T-cell line derived from the same animal prior to challenge (B). Homoduplexes formed by re-annealing of the carrier DNA strands are represented by a prominent band towards the bottom of each lane. Additional more slowly migrating bands in the upper part of the gel represent heteroduplexes formed from cDNA of expanded clonotypes expressing Vβ genes of the respective subgroup.