Figure 4.

Dose-dependent effects of self-complementary AAV serotype 2.5 vector (scAAV2.5)-bone morphogenetic protein-2 (BMP2) coating on new bone formation, allograft resorption and the polar moment of inertia of grafted femurs at 6 weeks. Micro-computed tomography (CT) scans were obtained of the femurs grafted with scAAV2.5-BMP2, and representative examples of the radiographic data generated from the (a) 10⁷, (b) 10⁸, (c) 10⁹, and (d) 10¹⁰ particles per allograft groups (n = 5) are shown. For each example, an image of the total bone volume (top left), graft volume (top center), new bone volume (top right), 2D cross-sections at 1 mm intervals (middle), and a graphic illustration of the polar moment of inertia (PMI) over the length of the allograft (bottom), are shown. Of note are the moth-eaten resorption surfaces of the allografts, which correspond to regions that are covered by new bone.