Skip to main content
. 2011 May 31;19(8):1529–1537. doi: 10.1038/mt.2011.104

Figure 4.

Figure 4

Glyceraldehyde-3-phosphate dehydrogenase (GAPD) suppression and evaluation of inflammatory response mediated by monoclonal antibody-streptavidin conjugate (mAb-SA) targeted polymeric micelles. (a) GAPD mRNA levels were assayed by quantitative reverse transcription (qRT)-PCR 48 hours after transfection of HeLa-R CD22+ cells with polymeric micelles bearing HD39-SA (CD22-targeted conjugate), BHV1-SA (nontargeted conjugate) or no conjugate containing 10 nmol/l small interfering RNA (siRNA) directed against GAPD or a negative control siRNA with no sequence homology to known human genes. Values are normalized to the housekeeping gene PPIA (Cyclophilin A) and relative to GAPD expression in untreated cells. Error bars represent the mean GAPD expression + s.d. of triplicate samples. Results are representative of three independent experiments. (b) Evaluation of expression of interferon response genes OAS and STAT1 by qRT-PCR after treatment of HeLa-R CD22+ cells with HD39-SA or BHV1-SA polymeric micelles containing 15 nmol/l siRNA or Poly I:C (0.5, 5, and 50 µg/ml) for 24 hours. Values are normalized to the housekeeping gene PPIA (Cyclophilin A). Error bars represent the mean + s.d. of triplicate samples. Results are representative of three independent experiments. OAS, 2′–5′ oligoadenylate synthetase; Poly I:C, polyinosinic–polycytidylic acid; STAT1, signal transducer and activator of transcription-1.