Skip to main content
. 2011 May 31;19(8):1529–1537. doi: 10.1038/mt.2011.104

Figure 5.

Figure 5

Dose response and 5′-RLM-RACE analysis of HeLa-R CD22+ cells treated with polymeric micelles bearing HD39-SA conjugate containing small interfering RNA (siRNA) directed against glyceraldehyde-3-phosphate dehydrogenase (GAPD) or a negative control siRNA with no sequence homology to known human genes. (a) Cells were harvested after 48 hours of treatment and analyzed using quantitative reverse transcription (RT)-PCR. Values are normalized to the housekeeping gene PPIA (Cyclophilin A) and relative to GAPD expression in untreated cells. Error bars represent the mean GAPD expression + s.d. of triplicate samples. (b) RNA was harvested from untreated HeLa-R CD22+ cells and HeLa-R CD22+ cells treated for 24 hours with polymeric micelles bearing HD39-SA conjugate at a dose of 15 nmol/l of GAPD siRNA or negative control siRNA. Relative GAPD expression normalized to the housekeeping gene PPIA was evaluated using quantitative RT-PCR. Error bars represent s.d. of triplicate samples. Specific detection of GAPD mRNA cleavage products using 5′-RLM-RACE assay was performed using RNA from each treatment group: GAPD siRNA (lane a), negative control siRNA (lane b), and untreated cells (lane c). PCR products were analyzed by agarose gel electrophoresis and run alongside a 100 base pair DNA ladder. The correct cleavage product indicated by the arrow has a size of 281 base pairs.