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. 2011 Feb 22;19(8):1457–1467. doi: 10.1038/mt.2011.10

Figure 3.

Figure 3

Delivery efficacy of stearyl-transportan 10 (TP10)/plasmid nanoparticles in different cell lines and importance of cell surface glucosaminoglycans (GAGs). (a) 5 × 104 U2OS, (b) U87, (c) 3 × 104 mouse embryonal fibroblast (MEF) and (d) 5 × 104 Chinese hamster ovary (CHO) cells were seeded 24 hours before experiment into 24-well plates. Cells were treated and analyzed as in Figure 2a,c, however, for the first 4 hours, transfections were also carried out in 10% serum containing medium, except in MEF cells. (e) 5 × 104 CHO and 5 × 104 GAG-deficient CHO cells were seeded 24 hours before experiment into 24-well plates. Cells were treated and analyzed as in Figure 2a,c. (f) Effect of the addition of chloroquine (CQ) to the transfection efficiency of stearyl-TP10/plasmid nanoparticles in CHO cells. Cells were treated and analyzed as in Figure 2a,c. The values represent the mean of at least three independent experiments performed in duplicate (mean ± SEM).