Table IV.

Kinetic Parameters of Monomeric Streptavidins Determined by SPR

	kon (M−1 sec−1)	koff (sec−1)	Kd (M)
mSA	1.2 × 105	1.3 × 10−2	1.0 × 10−7
mSA S52G	1.6 × 104	2.0 × 10−3	1.3 × 10−7
mSA S52G, R53S	1.9 × 104	2.0 × 10−3	1.0 × 10−7
mSA S52G, R53D	1.5 × 104	2.5 × 10−3	1.7 × 10−7